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Year Number of Results
1900 1
1933 1
1936 1
1937 1
1943 1
1945 1
1954 1
1962 1
1965 3
1966 4
1967 8
1968 7
1969 6
1970 2
1971 8
1972 6
1973 8
1974 13
1975 38
1976 49
1977 37
1978 56
1979 70
1980 67
1981 73
1982 84
1983 77
1984 110
1985 101
1986 106
1987 130
1988 139
1989 145
1990 174
1991 205
1992 222
1993 224
1994 276
1995 325
1996 305
1997 315
1998 327
1999 363
2000 423
2001 436
2002 402
2003 446
2004 556
2005 580
2006 625
2007 743
2008 732
2009 801
2010 824
2011 933
2012 1037
2013 1080
2014 1184
2015 1188
2016 1211
2017 1282
2018 1394
2019 1487
2020 1602
2021 1956
2022 2224
2023 2145
2024 998

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27,759 results

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Page 1
Classical Recombinant DNA Cloning.
Mikić A, Alomari A, Gowers DM. Mikić A, et al. Methods Mol Biol. 2023;2633:1-24. doi: 10.1007/978-1-0716-3004-4_1. Methods Mol Biol. 2023. PMID: 36853452
This comprises six linked stages in which (1) PCR is used to amplify a DNA region of interest that is then (2) digested with restriction enzymes, alongside a selected vector, to produce complementary ends crucial for the two molecules to be (3) ligated by an ATP-dependent …
This comprises six linked stages in which (1) PCR is used to amplify a DNA region of interest that is then (2) digested with restrict …
Selective effects of specificity inductions on episodic details: evidence for an event construction account.
Madore KP, Jing HG, Schacter DL. Madore KP, et al. Memory. 2019 Feb;27(2):250-260. doi: 10.1080/09658211.2018.1502322. Epub 2018 Jul 19. Memory. 2019. PMID: 30024835 Free PMC article.
We predicted that induction effects would be specific to episodic detail production on subsequent memory and imagination because these details assay critical elements of a constructed event. ...Induction did not increase detail generation on picture de …
We predicted that induction effects would be specific to episodic detail production on subsequent memory and imagination because thes …
The role of the hippocampus in memory and mental construction.
Sheldon S, Levine B. Sheldon S, et al. Ann N Y Acad Sci. 2016 Apr;1369(1):76-92. doi: 10.1111/nyas.13006. Epub 2016 Feb 5. Ann N Y Acad Sci. 2016. PMID: 26849289 Review.
We propose a new framework to answer these questions, which incorporates task demands that prompt hippocampal contributions to mental construction, the online formation of such mental representations, and how these demands relate to the functional organization of the hippo …
We propose a new framework to answer these questions, which incorporates task demands that prompt hippocampal contributions to mental con
Recombination map construction method using ONT sequence.
Chen Z, Xie L, Tang X, Zhang Z. Chen Z, et al. MethodsX. 2022 Dec 16;10:101969. doi: 10.1016/j.mex.2022.101969. eCollection 2023. MethodsX. 2022. PMID: 36590318 Free PMC article.
Here we detail a step-by-step approach to efficiently and accurately construct genome-wide recombination maps using ONT pooled sequencing data. The main contents include compression homopolymers and alignment; acquisition of high-quality variants; estimation of r
Here we detail a step-by-step approach to efficiently and accurately construct genome-wide recombination maps using ONT pooled …
TALEN-Based Knockout System.
Yoshida K, Treen N. Yoshida K, et al. Adv Exp Med Biol. 2018;1029:131-139. doi: 10.1007/978-981-10-7545-2_12. Adv Exp Med Biol. 2018. PMID: 29542086
Here, we introduce recent updates to the TALEN toolkit that facilitate detailed functional analysis of genes in ascidians....
Here, we introduce recent updates to the TALEN toolkit that facilitate detailed functional analysis of genes in ascidians....
Restriction endonucleases.
Roberts RJ. Roberts RJ. CRC Crit Rev Biochem. 1976 Nov;4(2):123-64. doi: 10.3109/10409237609105456. CRC Crit Rev Biochem. 1976. PMID: 795607 Review.
However, emphasis will be placed on the Class II group, which recognizes and cleaves a specific duplex DNA sequence. Their occurrence, purification, and characterization is discussed in detail. ...These uses include physical mapping of genomes and their transcripts, …
However, emphasis will be placed on the Class II group, which recognizes and cleaves a specific duplex DNA sequence. Their occurrence …
The art of vector engineering: towards the construction of next-generation genetic tools.
Nora LC, Westmann CA, Martins-Santana L, Alves LF, Monteiro LMO, Guazzaroni ME, Silva-Rocha R. Nora LC, et al. Microb Biotechnol. 2019 Jan;12(1):125-147. doi: 10.1111/1751-7915.13318. Epub 2018 Sep 26. Microb Biotechnol. 2019. PMID: 30259693 Free PMC article. Review.
When recombinant DNA technology was developed more than 40 years ago, no one could have imagined the impact it would have on both society and the scientific community. ...This technology has been continuously expanding and undergoing adaptations. Here, we provide a …
When recombinant DNA technology was developed more than 40 years ago, no one could have imagined the impact it would have on b …
Construction of a Z-DNA-Specific Recombinant Nuclease Zalphaalpha-FOK for Conformation Studies.
Lee SK, Kim YG. Lee SK, et al. Methods Mol Biol. 2023;2651:143-155. doi: 10.1007/978-1-0716-3084-6_10. Methods Mol Biol. 2023. PMID: 36892765
Development of FokI-based engineered nucleases has been a platform technology that enables creation of novel sequence-specific nucleases as well as structure-specific nucleases. Z-DNA-specific nucleases have been constructed by fusing a Z-DNA-binding domain t …
Development of FokI-based engineered nucleases has been a platform technology that enables creation of novel sequence-specific nucleases as …
Transgenic bioreactors.
Jänne J, Hyttinen JM, Peura T, Tolvanen M, Alhonen L, Sinervirta R, Halmekytö M. Jänne J, et al. Int J Biochem. 1994 Jul;26(7):859-70. doi: 10.1016/0020-711x(94)90078-7. Int J Biochem. 1994. PMID: 8063010 Review.
Although many human therapeutic proteins are currently produced in microbial fermentors using recombinant DNA techniques, it is obvious that microbial processing is not suitable for a large number of bioactive proteins owing to the inability of bacteria to carry out …
Although many human therapeutic proteins are currently produced in microbial fermentors using recombinant DNA techniques, it i …
Mnemonic construction and representation of temporal structure in the hippocampal formation.
Bellmund JLS, Deuker L, Montijn ND, Doeller CF. Bellmund JLS, et al. Nat Commun. 2022 Jun 23;13(1):3395. doi: 10.1038/s41467-022-30984-3. Nat Commun. 2022. PMID: 35739096 Free PMC article.
The hippocampal-entorhinal region supports memory for episodic details, such as temporal relations of sequential events, and mnemonic constructions combining experiences for inferential reasoning. ...Structural knowledge about time patterns, abstracted from differen …
The hippocampal-entorhinal region supports memory for episodic details, such as temporal relations of sequential events, and mnemonic …
27,759 results
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